KMID : 0043320100330122025
|
|
Archives of Pharmacal Research 2010 Volume.33 No. 12 p.2025 ~ p.2031
|
|
Absence of drug interaction between Hwang-Ryun-Hae-Dok-Tang and Phenolsulfonphthalein
|
|
Yi Hong-Jae
Oh Ju-Hee Lee Young-Joo
|
|
Abstract
|
|
|
Hwang-Ryun-Hae-Dok-Tang (HT; a standardized herbal formula consisting of extracts from Coptidis Rhizoma, Scutellariae Radix, Phellodendri Cortex, and Gardeniae Fructus) was reported to modulate a function of multidrug resistance associated protein 2 (Mrp2) in vitro. The aim of this study was to assess the in vivo pharmacokinetic interactions between HT and phenolsulfonphthalein (PSP), a typical model Mrp2 substrate eliminated via bile through Mrp2 in rats. Rats received intravenous PSP (0.8 mg/kg) followed by either a single oral dose of HT (0.42 g/kg) or multiple oral doses of HT (0.42 g/kg for 7 days). The effect of HT treatment was also investigated at a steady-state after intravenous PSP infusion. In contrast to previous in vitro results, in this study, we found that the HT-treated and control groups did not show any significant difference in the plasma PSP concentration and pharmacokinetic parameters, including area under the plasma concentration-time curve (AUC; control: 118 ¡¾ 19, single dose: 116 ¡¾ 40, and multiple dose: 137 ¡¾ 4, in mg/(min¡¤mL)) and biliary clearance (control: 3.15 ¡¾ 0.69, single dose: 2.59 ¡¾ 1.11, and multiple dose: 2.53 ¡¾ 0.65, in mL/(min¡¤kg)). However, cyclosporine A (5 mg/kg, an inhibitor of Mrp2) significantly decreased the AUC and biliary clearance of PSP. The steady-state plasma concentration and biliary clearance of PSP-were also similar between the groups. Taken together, our results suggest that HT may not be affected by Mrp2-mediated herb-drug interaction in vivo.
|
|
KEYWORD
|
|
Herb-drug interaction, Pharmacokinetics, Hwang-Ryun-Hae-Dok-Tang, Phenolsulfonphthalein, Biliary excretion
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|